The stat proteins function both as cytoplasmic signal transducers and as activators of transcription.
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin.
Phosphorylation at Tyr-694 is required for Stat5A activation.
Stat5 has been found to be constitutively active in some leukemic cell types.
Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility.
Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types.
For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity：Recognizes human STAT 5, phosphorylated at Tyr694. Species Crossreactivity: mouse and rat. Detects a 92kD band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.
Clone # USB：M148
Isotype：IgG1
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acid residues around tyrosine 694 of human Stat5A, coupled to KLH. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
