Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine.
Mature porcine IFN-gamma exists as a noncovalently linked homodimer of 20‑25kD variably glycosylated subunits.
It shares 72%‑79% amino acid sequence identity with bovine, canine, equine, and feline IFN-gamma and 41%‑57% with cotton rat, human, mouse, rat, and rhesus IFN-gamma.
IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits.
Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction.
IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells.
It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells.
It also exhibits antiviral, anti-proliferative, and apoptotic effects.
In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation.
The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: use as a capture antibody with 052076 as detectionOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes porcine IFN‑ gamma
Clone # USB：24I2
Isotype：IgG2a
Calc Applications Abbrev：E
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Ser21-Lys166 from porcine IFN gamma, expressed in E. coli.
