83-5981-26　Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD) 100ug　052074

特徴

• Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein.
• It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis.
• Uromodulin forms high molecular weight oligomers that line the kidney tubules.
• Mouse Uromodulin is GPI-linked.
• Its proprecursor is 619aa in length.
• It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642).
• There are multiple splice variants.
• One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642.
• Over aa25-614, mouse Uromodulin shares 78% and 89%aa identical to human and rat Uromodulin, respectively.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml using mouse kidney tissueImmunohistochemistry: 8-25ug/ml using immersion fixed frozen sections of mouse kidneyOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.