Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily.
It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2).
Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3).
Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2).
Mature monomeric BMP-2 is an 18kD glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4).
Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.
It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs.
BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5).
BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7).
It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10).
BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11).
BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA (detection): 0.5-2ug/ml with 052066 as capture antibodyOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant
Form：Supplied as a lyophilized powder from PBS, sodium chloride, BSA. Labeled with Biotin. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human BMP-2. ≤10% cross-reactivity with recombinant human BMP‑4, BMP‑2/BMP‑7 heterodimer. Does not react with recombinant human BMP-3, -5, -6, -7, TGF-beta 1, TGF-beta 2, or TGF-beta 3
Clone # USB：24H4
Isotype：IgG1
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln283-Arg396 from human BMP-2, expressed in CHO cells
