CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105kD member of a novel family of EGF domain-containing proteins.
It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization or cell-matrix adhesion.
Mature human CRTAC1 is 634 amino acids (aa) in length.
It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605).
Multiple splice forms exist.
There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661.
Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1.
This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution.
In this case, there is 95% aa identity between mouse and human.
Applications:Suitable for use in Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 8-25ug/ml with immersion fixed human mesenchymal stem cells differentiated into chondrocytesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human CRTAC1.
Clone # USB：24H1
Isotype：IgG2b
Calc Applications Abbrev：IC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser28-Cys661 from human CRTAC1, expressed in NS0 cells
