Erythropoietin (EPO) is a 34kD glycoprotein hormone in the type I cytokine family and is related to thrombopoietin.
Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species.
Glycosylation of EPO is required for biological activities in vivo.
Mature human EPO shares 75%-84% amino acid sequence identity with bovine, canine, equine, feline, mouse, sheep, porcine, and rat EPO.
EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules.
It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain.
EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R).
EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells.
Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation.
Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations.
Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha.
EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
Applications:Suitable for use in Neutralization.
Other applications not tested.
Recommended Dilution:Neutralization: Measured by its ability to neutralize Erythropoietin induced proliferation in the TF‑1 human erythroleukemic cell line.
The Neutralization Dose (ND50 ) is typically 0.02-0.2ug/ml in the presence of 0.3units/ml recombinant human ErythropoietinOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human Erythropoietin/EPO
Clone # USB：24G4
Isotype：IgG1
Calc Applications Abbrev：N
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala28-Arg193 from human Erythropoietin, expressed in CHO cells
