CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates.
It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma.
The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells.
CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested
Recommended Dilution:Flow Cytometry: 10ul labels 10e6 cells in 100ul or 100ul whole bloodOptimal dilution determined by the researcher.
Excitation Laser:Red (633nm)
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human CD146. Species Crossreactivity: canine, rabbit and mouse. Does not react with rat.
