BoNT-D (Botulinum neurotoxin serotype D) is produced by Clostridium botulinum and inhibits acetylcholine release from neuromuscular junctions.
Notably, BoNT-D is not toxic to human cells.
The 1276 amino acid (aa) BoNT-D precursor is cleaved to generate a disulfide-linked dimer of the 50‑55kD light chain peptidase and the 100kD heavy chain.
The heavy chain contains receptor binding and pore forming domains.
The light chain translocates through this pore to the target cell cytosol where it cleaves synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane.
Within aa862-1276 of the heavy chain, BoNT-D shares 31%, 33%, and 41% aa sequence identity with BoNT-A, -B, and -C1 heavy chains, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using recombinant C.
botulinum BoNT-D Heavy ChainOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes C. botulinum BoNT-D Heavy Chain. ~10% reactivity with recombinant C. botulinum BoNT-C1 Heavy Chain. No cross‑reactivity with the heavy chains of BoNT-A, -E, or -G or the light chains of BoNT-A, -B, -C1, -D, -E, -F, -G.
Clone # USB：24G3
Isotype：IgG2b
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to Asn862-Glu1276 from C. botulinum Neurotoxin Type D Heavy Chain, expressed in E. coli
