Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2).
BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins.
The 50kD light chain contains the catalytic domain, whereas the 100kD heavy chain contains an internal translocation domain and a receptor binding domain (3).
BoNTs are the most potent protein toxins for humans.
As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using recombinant Botulinum Neurotoxin Type B Light ChainOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes C. botulinum BoNT-B Light Chain. No cross‑reactivity with recombinant Light Chains of BoNT-A, -C1, -D, -E, -F, or -G
Clone # USB：24G2
Isotype：IgG1
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to Pro2-His428 from C. botulinum BoNT-B Light Chain, expressed in E. coli
