83-5980-52　Botulinum Neurotoxin Type A Light Chain (C. botulinum BoNT-A Light Chain, BoNTA Light Chain, BoNT-A Light Chain) 100ug　051997

特徴

• Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2).
• BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins.
• The 50kD light chain contains the catalytic domain, whereas the 100kD heavy chain contains an internal translocation domain and a receptor binding domain (3).
• BoNTs are the most potent protein toxins for humans.
• As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4).
• E.
• coli-expressed recombinant light chains are active proteases.
• However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
• Applications:Suitable for use in Immunoprecipitation and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml using recombinant C.
• botulinum BoNT-A Light ChainImmunoprecipitation: 25ug/ml using cell lysates spiked with recombinant C.
• botulinum BoNT-A Light ChainOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.