Claudin 4 is a multipass membrane protein in the claudin family of tight junction proteins.
Claudin 4 is a 209 amino acid molecule with four transmembrane segments.
It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development.
It is also upregulated in several carcinomas.
Phosphorylation of claudin 4 regulates paracellular epithelial permeability.
Human claudin 4 shares 83% aa sequence identity with mouse and rat claudin 4.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul/10e6 cells in PC-3 human prostate cancer cell line
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Claudin 4. Recognizes an epitope in the first extracellular loop (between Met29-Arg81). Stains human Claudin 4 transfectants but not irrelevant transfectants.
Clone # USB：7H177
Isotype：IgG2a
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val209 from human Claudin 4 expressed in NS0 cells. Species Sequence Homology: mouse and rat, 83%
