Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells.
MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development.
An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain.
Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25-1ug/10e6 cells using PC-12 rat adrenal pheochromocytoma cell line
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes rat CD146 (MCAM). Does not crossreact with recombinant human MCAM, BCAM or ALCAM.
Clone # USB：7H217
Isotype：IgG2a
Calc Applications Abbrev：FC
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg19-Lys560 from rat CD146 expressed in NS0 cells. Species Sequence Homology: human; 72%, mouse; 90%
