PCLO (piccolo, also known as aczonin) is a large (more than 400kD) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates.
It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B).
In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels.
It is involved in neurotransmitter release and insulin secretion.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Western Blot: A small splice variant of PCLO can be analyzed, however, the whole protein is too large (over 400kD) to be successfully processed by SDS-PAGE
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human PCLO.
Clone # USB：PCLO-01
Isotype：IgG1
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PCLO
