Structural polyprotein (UniProt: Q8QZ72; also known as p130) is encoded by the MAYVgp3 gene (Gene ID: 935141) in Mayaro virus.
Following its synthesis it is cleaved into the following six chains: Capsid protein (aa 1-258), Precursor of protein E3/E2 (aa 259-746), Assembly protein E3 (aa 259-324), Spike glycoprotein E2 (aa 325-746), 6K protein (aa 747-806), and Spike glycoprotein E1 (aa 807-1242).
Mayaro virus (MAYV) is a mosquito-transmitted arthritogenic alphavirus in the Togaviridae family of positive-stranded RNA viruses.
Its envelope glycoproteins associate in the endoplasmic reticulum and form a heterotrimer comprised of the E3, E2, and E1 proteins.
E2 is a single-pass type I membrane protein that plays a role in viral attachment to target host cell, by binding to the cell receptor.
It is synthesized as a p62 precursor (precursor of protein E3/E2), which is then processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer.
The p62-E1 heterodimer is shown to be stable, whereas E2-E1 is unstable and dissociates at low pH.
It has been reported that p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface.
The C-terminus region of E2 contains a transitory transmembrane region that is disrupted by palmitoylation and results in reorientation of the C-terminal tail from lumenal to cytoplasmic side.
Clone MAY-115 offers protection against lethal effects of MAYV in a murine model when injected one day prior to virus inoculation.
However, its protective effects are significantly reduced when injected two days post infection.
Applications:Suitable for use in ELISA and Neutralization.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes E1 glycoprotein in Mayaro virus.
Clone # USB：MAY-115
Isotype：IgG2a,k
Calc Applications Abbrev：E N
Immunogen：Mayaro virus, CH strain and boosted with recombinant MAYV E2 protein mixed with incomplete Freund's adjuvant. Epitope: N-terminal extracellular domain
