83-5980-23　HSP60 100ul　051965

特徴

• The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range.
• They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly.
• The need for chaperones is much greater if a cell or tissue is stressed by heat, and so these proteins become heavily up regulated.
• The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP60 has an apparent molecular weight of 60kDa.
• It is an abundant protein in mitochondria and is typically responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix 1,2).
• In addition to its role as a heat shock protein, HSP60 plays an important role in the transport and maintenance of mitochondrial proteins as well as the transmission and replication of mitochondrial DNA (3,4).
• HSP60 has been implicated in the initiation and/or progression of some subtypes of cardiovascular disease (CVD), implying its potential as a biomarker with applications for diagnosis, assessing prognosis and response to treatment, as well as for preventing and treating CVD (5).
• HSP60 appears to be unusually immunogenic, frequently generating autoantibodies in humans and other species (e.g.
• 6).
• The HSP60 protein presumably released from damaged or degenerated cells is also a strong inducer of the innate immune system (7).
• Applications:Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:10,000Immunofluorescence (IC): 1:5000Immunohistochemistry: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.