83-5980-18　Heparan Sulfate Proteoglycan (Perlecan) 100ug　051960

特徴

• Perlecan is one of the extracellular matrix forms of heparan sulfate proteoglycans (HSPGs).
• It is a large multidomain and binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules.
• Perlecan is synthesized by vascular endothelial and smooth muscle cells and deposited in the extracellular matrix within all mammalian tissues.
• HSPGs differ in their protein cores that are thought to determine the location of HSPG in either the cell membrane (syndecan and glypican) or the extracellular matrix (perlecan and dystroglycan).
• HSPGs found in the brain are dystroglycan, N-syndecan glypican, and perlecan.
• The composition of HS isolated from brain differs significantly from that of other organs and is developmentally regulated.
• Thus, differences in protein cores, disaccharide composition, the extent and position of sulfation, and the number of GAG chains diversify chemical structure and functions of HSPGs.
• The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:300 detected Perlecan in fresh-frozen normal human tonsil tissue
• Optimal dilutions to be determined by the researcher.
• Positive Control:Fresh-frozen normal human tonsil tissue
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.