Low-density lipoprotein receptor-related protein 2 (UniProt P98164; also known as Calcium sensor protein, Glycoprotein 330, gp330, Heymann nephritis antigen homolog, LRP-2, Megalin) is encoded by the LRP2 (also known as DBS) gene (Gene ID 4036) in human.
Megalin/LRP-2 is a 600kD type I membrane receptor initially produced with a signal peptide sequence (aa1-25).
The mature receptor has a large extracellular region (aa26-4423), a transmembrane segment (aa4424-4446), and cytoplasmic tail (aa4447-4655).
Megalin belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the very-low-density lipoprotein (VLDL) receptor, the LDLR-related protein (LRP), and the apolipoprotein E receptor 2 (ApoER2).
The overall extracellular structural features are similar among all LDLR family members.
Megalin contains four clusters of cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding.
These are separated by 17 EGF-like repeats and eight cysteine-poor spacer regions that contain YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments.
Megalin is a multiligand, endocytic receptor expressed primarily in the apical membranes of polarized epithelial cells.
In addtion to functioning alone, megalin is also reported to interact with another structurally distinct endocytic receptor cubilin in several tissues where they are co-expressed.
The internalization of several cubilin ligands is reported to be strongly facilitated in the presence of megalin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1:1000 detected LRP-2/Megalin in human kidney tissue
Western Blot: 0.5ug/ml detected LRP-2/Megalin in 10ug of human kidney tissue lysate using heat-induced epitope retrieval (HIER)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human LRP2.
Clone # USB：10D5.1
Isotype：IgG1,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：GST-tagged recombinant protein corresponding to the LDL-receptor class A repeats 19-21 of human LRP-2/Megalin.
