Gangliosides are sialic acid-containing oligoglycosylceramides.
They are classified based on the number of sialic acid residues per molecule.
GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, and GQ contain four sialic acid residues.
Sialyltransferase-catalyzed 2-3 sialylation of the galactose of the trisaccharide Gal 1-4Glc 1-1 Cer produces GM3, which can be further sialylated to produce GD3 with two sialic acid on the galactose.
GD3 is found in melanocytes and other cells that originate in the neuroectoderm.
It is expressed almost exclusively among gangliosides in the early stage of the brain development of mice and rats.
It is also detected in the T cell malignant leukocytes, such as acute lymphoblastic leukemia cells, adult T cell leukemia cells, and in activated normal human T lymphocytes.
GD3 is found in almost all melanomas and is considered as an important biomarker for malignant melanoma.
GD3 positive cells show markedly increased cell growth and invasive characteristics.
Suppression of GD3 expression with antisense GD3 synthase cDNA is shown to reduce tumor cell phenotypes such as growth, migration, metastasis, and angiogenesis in F11 cells.
Intra-cytoplasmic domain of neogenin is reported to act as a driver to promote various cancer phenotypes.
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Affinity Binding Assay and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Flow Cytometry: 0.2ul detected GD3 immunoreactivity on the surface of one million SK-MEL-28 human skin melanoma cells
Immunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃.
For long-term storage, aliquot and store at 4℃.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes Diasialoganglioside GD3. Immunostained neurites of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice. Species Crossreactivity: mouse and rat. Target glycolipid is not species-specific.
