Streptavidin is a tetrameric protein purified from Streptomyces avidinii.
It is widely used in numerous molecular biological protocols due to its strong affinity for biotin.
NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:ELISA: 0.001-0.002ug/mlWestern Blot: 0.1-0.5ug/mlImmunofluorescence (IC): 1-3ug/mlFlow Cytometry: 1ug/mlImmunoprecipitation: 1-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes NWSHPQFEK tags localized at the N-terminal and C-terminal region of Strep II-tagged fusion proteins.
