Trypsin-1 (UniProt: P07477; also known as EC:3.4.21.4, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I) is encoded by the PRSS1 (also known as TRP1, TRY1, TRYP1) gene (Gene ID: 5644) in human.
Trypsin is pancreatic serine protease that is synthesized as an inactive precursor known as trypsinogen that is processed through the Golgi apparatus and packaged into zymogen granules for secretion by exocytosis.
Trypsin-1 is synthesized with a signal peptide (aa1-15) and a propeptide (trypsin activation peptide; aa16-23) that are subsequently cleaved off to generate the mature form that can be further cleaved and processed into alpha-trypsin chain 1 (aa24-122) and alpha-trypsin chain 2 (aa 23-247).
Trypsin can be present in a single-chain and a two-chain form, produced by proteolytic cleavage after arginine 122.
Trypsin can also undergo sulfation at tyrosine 154 that increases its selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion.
Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acid lysine or arginine, except when either is followed by proline.
Applications:Suitable for use ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1:100-1:500; formalin fixed, paraffin embedded human tissues; 5m serial sections; blocking solution 0.5% serum in PBS; overnight incubations; enzymatic detection
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
