PTH (Parathyroid Hormone) is a critical hormone in the regulation of Ca++ homeostasis.
The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone.
Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively.
Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation.
PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis.
C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure.
PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus.
PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23.
In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium.
Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production.
PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells.
TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone.
PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production.
In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion.
PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human parathyroid glandWestern Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human PTHLH.
Clone # USB：24F1
Isotype：IgG2b
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser32-Gln115 from human PTH expressed in E. coli.
