Proteoglycans (PG), present in all tissues, are one of the best-studied classes of matrix molecules.
Over 50 members of PG have been characterized and studied for their biological significance.
PG consist of a protein core to which glycosaminoglycan chains are covalently linked.
Glycosaminoglycans (GAGs) are natural heteropolysaccharides that are present in every mammalian tissue.
They are composed of repeating disaccharide units that consist of either sulfated or non-sulfated monosaccharides.
Their molecular size and the sulfation type vary based on tissue type.
The major types of GAGs found in mammalian tissues are: hyaluronan, chondroitin sulfate, and dermatan sulfate, heparin and heparan sulfate, and keratan sulfate.
GAG chains are covalently bound to serine residues of the PG protein core via a tetrasaccharide linkage, consisting of xylose, two galactose residues and glucuronic acid.
This clone (PG-4) recognizes antigen that is associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and the surfaces of cultured human adult keratinocytes.
Treatment of PG with chondroitinases is reported to abolish its immunoreactivity for all antigens.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (paraffin): 1:250 detected Glycosaminoglycan in human skin tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human glycosaminoglycan in skin cells. Recognizes native epitopes located within glycosaminoglycan chains and is detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin and Biglycan) proteoglycans. Species Crossreactivity: chicken and shark.
Clone # USB：PG-4
Isotype：IgM,k
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ch Hu
Immunogen：Crude membrane fractions from adult human dermal fibroblasts from breast skin.
