Plasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human.
PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell.
PMCAs serve as the main off switch for calcium signals in all types of cells.
Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties.
PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization.
The localization of different PMCAs is regulated in various complex, cell type-specific ways.
PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung.
PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons.
PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms.
Applications:Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:250 detected pan-PMCA in human pancreas and human kidney tissue
Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes all isoforms of human Plasma membrane calcium-transporting ATPase (PMCA). Species Crossreactivity: chicken and rat
Clone # USB：5F10
Isotype：IgG2a,k
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Ch Hu Rt
Immunogen：Purified human erythrocyte calcium ATPase.
