LINE-1 retrotransposable element ORF2 protein (UniProt: O00370; also known as EC:2.7.7.49, ORF2p endonuclease, ORF2p) is encoded by the Orf2p gene (Gene ID: 107987054) in human.
Full length long interspersed element-1 (L1) is an autonomous non-long terminal repeat retrotransposon that contains two open reading frames encoding ORF1p and ORF2p proteins.
L1 is reported to be responsible for all of the retrotransposon-induced genomic instability in the human genome and is the only active source of the functional ORF1 and ORF2 proteins required for mobilization of LINEs, SINEs, and SVA elements.
The human ORF2p, a 149kD protein, contains three domains: an N-terminal endonuclease domain, a reverse transcriptase domain, and C-terminal domain with putative RNA binding activity.
Its endonuclease activity allows the introduction of nicks in the chromosomal target DNA and is known to cleave DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome.
Human and mouse ORF2p is reported to exhibit a high degree of sequence homology.
Applications:Suitable for use in ELISA, Inhibtion and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 detected LINE1 ORF2 in lysate from HEK293 cells overexpressing human ORF2p EN
Optimal dilutions to be determined by the researcher.
Molecular Weight:~32kD for truncated recombinant endonuclease domain; 149.01kD calculated for full-length protein.
Uncharacterized bands may be observed in some lysate(s).
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes sequences within the endonuclease domain of the human ORF2 protein. It exhibits specificity to the human ORF2p and can detect the full-length and truncated ORF2 proteins expressed in mammalian cells.
Clone # USB：1G4E11
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human protein containing an ORF2p endonuclease, fused to His-Tag at N-terminal.
