83-5980-06　NFATc1 (Nuclear Factor of Activated T-Cells, Cytoplasmic 1, NFAT Transcription Complex Cytosolic Component, NFAT2, NFATc) 100ug　051942

特徴

• The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex.
• The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4.
• All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response.
• NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells.
• In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm.
• Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.
• Applications:
• Suitable for use in Western Blot and ChIP.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1ug/mlChIP: 1:100-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.