Sialyl Lewis X, also known as sialyl LeX and SLeX, is a tetrasaccharide carbohydrate that is usually attached to O-glycans on the surface of cells.
It is known to play a vital role in cell-to-cell recognition processes.
Sialyl Lewis X is one of the most important blood group antigens and is displayed on the terminus of glycolipids that are present on the cell surface.
The Sialyl Lewis X determinant, E-selectin ligand carbohydrate structure, is constitutively expressed on granulocytes and monocytes and mediates inflammatory extravasation of these cells.
Resting T and B lymphocytes lack its expression and are induced to strongly express sialyl Lewis X upon activation.
The Sialyl Lewis X determinant is expressed preferentially on activated Th1 cells but not on Th2 cells.
Applications:
Suitable for use in Flow Cytometry.
Other applications have not been tested
Recommended Dilutions:Flow Cytometry: 5ul/10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human Sialyl Lewis X (dimeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i).
Clone # USB：FH6
Isotype：IgM,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Purified 6B fucoganglioside absorbed to Salmonella minnesota.
