The mecA gene allows a bacterium to be resistant to antibiotics such as methicillin, penicillin, erythromycin, tetracycline and other penicillin-like antibiotics.
The most commonly known carrier of the mecA gene is the bacterium known as MRSA.
It is also found in Staphylococcus aureus and Streptococcus pneumoniae strains resistant to penicillin-like antibiotics.
In Staphylococcus species, mecA is spread on the SCCmec genetic element.
The mecA gene does not allow the ringlike structure of penicillin-like antibiotics to attack the enzymes that help form the cell wall of the bacterium (transpeptidases), and hence the bacteria is allowed to replicate as normal.
The gene encodes the protein PBP2A (Penicillin binding protein 2A).
PBP2A has a low affinity for beta-lactam antibiotics such as methicillin and penicillin.
This enables transpeptidase activity in the presence of beta-lactams, preventing them from inhibiting cell wall synthesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:40,000Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography from tissue culture supernatant.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes S. aureus PBP2a. Does not show any cross reactivity with unrelated antigens in ELISA.
Clone # USB：24E7
Isotype：IgG1
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to PBP2a.
