83-5979-60　TrkC (Tyrosine Kinase Receptor C) 500ug　048946

特徴

• The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system.
• Binding to and activating two unrelated receptor types mediate the biological activities of the neurotrophins.
• These receptors include the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases.
• P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16.
• It binds all neurotrophins and transduces cellular signaling pathways that may synergize or antagonize those activated by the Trk receptors.
• Three Trk family proteins, TrkA, TrkB, and TrkC, have been identified.
• TrkA preferentially binds NGF and NT-3, TrkB preferentially binds BDNF, NT-3, and NT-4/5, and TrkC preferentially binds NT-3.
• All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain.
• Natural splice variants of the different Trks lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain have been described.
• At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC exhibiting 94% amino acid sequence identity.
• The proteins also exhibit cross- species activity.
• Although TrkC is widely expressed, the highest levels are found in the nervous system.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry: 8-25ug/ml in perfusion fixed frozen sections of rat spinal cord Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.