83-5979-58　ATE1 (Arginyltransferase 1, Arginine-tRNA--Protein Transferase 1) 100ug　048943

特徴

• ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway.
• ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins.
• The arginylation is required for effective ubiquitin mediated protein degradation.
• ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation.
• This system disposes of malformed or misdirected proteins and protein fragments.
• ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized.
• ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation.
• Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/ml detected ATE1 in 10ug of HepG2 cell lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.