83-5979-56　Sulf-2 (Extracellular Sulfatase Sulf-2, SULF2, KIAA1247) 100ug　048941

特徴

• Sulf-2 is an endoplasmic reticulum enzyme that is involved in sulfur metabolism which is important in bone, cartilage, and skeletal development.
• Sulf-2 is also important in neurological situations including myelination and glial cell differentiation and GDNF activity.
• Sulf-2 activity is also found in the kidney and important in glomerular basement membrane development and glomerular function.
• Finally, Sulf-2 is involved in heparan sulfate metabolism.
• Sulf-2 removes sulfate moieties on heparan sulfate proteoglycans which in turn modulate the heparin binding growth factor signaling activity of the cell.
• Sulf-2 also impacts extracellular matrix interactions and it is important during development and growth, as well as Wnt signaling and VEGF production by endothelial cells during vascularization.
• In disease, Sulf-2 expression in cancer is upregulated and inhibition of Sulf-2 limits cancer progression and showed increased apoptosis within tumors, thus Sulf-2 activation is being examined as a potential anti-cancer therapy.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 4ug/ml detected Sulf-2 in Sulf-2 transfected HEK293 cells, but not in HEK293 cells transfected with an empty vector or Sulf-1
• Western Blot: 0.5ug/ml detected Sulf-2 in 10ug of MCF-7 conditioned media
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.