83-5979-46　Interleukin 4 Receptor alpha (IL-4R alpha, CD124) 100ug　592484

特徴

• Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex.
• In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1.
• IL-4 R is expressed by B cells, T cells, monocytes and macrophages.
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry and Neutralization.
• Other applications not tested.
• Recommended Dilutions:Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line.
• The ND50 is typically 3-6ng/mL in the presence of 0.2ng/mL recombinant human IL‑4
• Flow Cytometry: 0.25ug/10e6 cellsImmunohistochemistry: 8-25ug/ml for immersion fixed paraffin-embedded sections of human lymph nodeImmunocytochemistry: 5-15ug/ml for immersion fixed HDLM-2 human Hodgkin's lymphoma cell lineWestern Blot: 1ug/ml detects human IL-4R under non-reducing conditionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.