83-5979-37　Polypyrimidine Tract Binding Protein (PTB, PTBP1) 100ug　592475

特徴

• Polypyrimidine tract-binding protein 1 (UniProt P26599; also known as 57 kDa RNA-binding protein PPTB-1, Heterogeneous nuclear ribonucleoprotein I, Heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, PTB, RNA-binding protein) is encoded by the PTBP1 (also known as HNRNP-I, HNRNPI, HNRPI, PTB, PTB2, PTB3, PTB4, PTB-1, PTB-T) gene (Gene ID 5725) in human.
• The serine–arginine rich (SR) proteins and the heterogeneous nuclear ribonucleoproteins (hnRNPs) are the most typical families of RBPs involved in splicing regulation.
• SR proteins generally play a positive role in exon recognition by binding exonic and intronic enhancers near the alternative splice sites.
• On the other hand, hnRNPs often bind to silencer elements and interfere with the recruitment of spliceosome components or positive regulators.
• PTB, also known as hnRNP I, belongs to an hnRNP family of RNA-binding proteins and is involved in several aspects of cellular mRNA metabolism including splicing, RNA stability, and internal ribosome entry site (IRES)-mediated translation of viral and cellular mRNAs.
• PTB functions as a splicing repressor by preventing recruitment of U2AF65 or correct base pairing of U2 snRNP, or by affecting the interaction of U1 snRNP with other components of the spliceosome.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml detected PTB in 10ug of HeLa cell lysate
• Immunocytochemistry (IF): 1:500 detected PTB in HeLa, HUVEC, and NIH/3T3 cells.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.