83-5979-35　Claudin 2 (CLDN2, SP82) 100ul　592473

特徴

• Claudin-2 (UniProt: P57739; also known as SP82) is encoded by the CLDN2 (also known as PSEC0059, SP82, UNQ705/PRO1356) gene (Gene ID: 9075) in human.
• Claudin-2 is a multi-pass membrane protein with three cytoplasmic domains, two extracellular domains, and four transmembrane domains.
• It can exist as homopolymer or as a heteropolymer with other claudins mediated through the first extracellular domain (aa29-81).
• These interactions are achieved largely through electrostatic interactions with Asp65 and with somewhat weaker interaction with electrons of the Tyr67.
• Claudin-2 is expression has been reported in liver, kidney, and small intestine.
• It is a component of epithelial tight junctions (TJ) that plays a key role in TJ-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
• Its expression is shown to be enhanced in subjects with inflammatory bowel disease.
• Treatment with TNF-a is also reported to upregulate the expression of Claudin-2 via PI3-kinase signaling that reduces transepithelial electrical resistance (TEER).
• 592473 is shown to reduce TNF-a-induced reduction in TEER and restore TJ integrity.
• (Ref.: Takigawa, M., et al.
• (2017).
• J.
• Pharmacol.
• Exp.
• Ther.
• 363(3); 444-451).
• Applications:Suitable for use in Flow Cytometry and Inhibition Assays.
• Other applications have not been tested.
• Recommended Dilutions:Flow Cytometry: 1ug detected Claudin-2 in 10e6 cells HT1080 cells transfected with human Claudin-2
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.