Prelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human.
It is cleaved into Lamin-A/C (also known as 70 kDa Lamin, Renal carcinoma antigen NY-REN-32).
Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin.
Lamin A and C are present in equal amounts in the lamina of mammals.
Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics.
Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A.
Cleavage of 15 residues (aa647-662) by ZMPSTE24/FACE1 generates the final protein product.
Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery.
Prelamin-A/C can accelerate smooth muscle cell senescence.
It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system.
Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body.
Applications:Suitable for use in Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry (IF): 1:1000 detected Lamin A/C in HeLa cells
Western Blot: 0.2ug/ml detected Lamin A/C in HeLa cell lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Lamin A/C and multiple species in the nuclear interior. Targets the Rod domain of Lamin A/C. Species Crossreactivity: hamster, monkey and mouse
Clone # USB：2A1
Isotype：IgG2b,k
Calc Applications Abbrev：IC IF IP WB
Calc Crossreactivity：Hm Hu Mk Mo
Immunogen：Purified protein corresponding to the rod domain for human Lamin A/C.
