HLA class I histocompatibility antigen, alpha chain G (UniProt: P17693; also known as HLA G antigen, MHC class I antigen G) is encoded by the HLA-G (also known as HLA-6.0, HLAG) gene (Gene ID: 3135) in human.
HLA-G is a single-pass type I membrane glycoprotein that serves as marker of extravillous cytotrophoblasts in placenta and plays a central role in molecule maternal tolerance of the semiallogeneic fetus.
It is involved in the presentation of foreign antigens to the immune system.
HLA-G is a heterodimer of an alpha chain and a beta chain.
Severn different isoforms of HLA-G have been described that are produced by alternative splicing and their distribution is temporally affected during pregnancy in placenta and fetal tissues.
HLA-G expression has been reported in renal cell carcinoma where it is exclusively restricted to tumor cells.
Its expression is thought to be involved in the impairment of the anti-tumor immunity or resistance to immunotherapeutic agents.
HLA-G is synthesized with a signal peptide (aa1-24), which is subsequently cleaved off to generate the mature form, which contains an extracellular domain (aa25-308), a transmembrane domain (aa309-332), and a short cytoplasmic tail (aa333-338).
Applications:Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50 detected HLA-G in human spleen tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human HLA-G. It recognizes an epitope with in 23 amino acids from the alpha1 region of all HLA-G isoforms.
Clone # USB：4H84
Isotype：IgG1,k
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human MHC class I antigen G. The immunogen sequence is conserved in all isoforms.
