PSD-95 is a member of proteins located at a specialized postsynaptic membrane region, called the postsynaptic density (PSD).
PSD-95 is the most abundant scaffold protein specifically enriched in the PSD.
It contains three PDZ domains, an SH3 domain and a guanylate kinase (GuK)-like domain.
Through its PDZ domains, PSD-95 assembles various synaptic components at the PSD including intracellular signaling molecules (e.g.
SynGAP and kalirin-7), ion channels (e.g.
stargazin/AMPA receptors [AMPARs] and NMDA receptors) and cell adhesion molecules (e.g.
neuroligin).
PSD-95 plays a primary role in synaptic development and maturation and is regulated by palmitoylation at its N-terminal cysteine residues leading to its postsynaptic targeting.
Palmitoylation of PSD-95 is triggered by DHHC2, a plasma membrane-inserted palmitoylating enzyme.
Palmitoylated PSD-95 is almost exclusively localized at excitatory synapses in neurons.
048732 is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Produced without the use of animals.
Purified from HEK 293 cell culture supernatant.
Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry (fixed with methanol): 1:1000Immunohistochemistry (fixed with acetone; does not work with paraformaldehyde): 1:500Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from animal free supernatant. ≥95% (SDS-PAGE)
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human, mouse and rat palmitoylated PSD-95.
