Legionella are flagellated Gram-negative bacteria that belong to the class of gamma-proteobacteria.
They are ubiquitously present in water habitats, including natural and man-made reservoirs where they infect and parasitize protozoa.
In the host, a specialized compartment is formed, that enables replication of Legionella.
When humans inhale aerosolized water contaminated with Legionella, alveolar macrophages can be infected, which may lead to a life-threatening pneumonia called Legionnaires’ disease.
Due to the universal distribution of Legionella in water and their potential threat to human health, the Legionella concentration in water for human use must be strictly monitored, which is difficult since the standard detection still relies on lengthy cultivation and analysis of bacterial morphology.
Specific and fast detection of L.
pneumophila based on a highly stable antibody-based assay would be potentially useful.
This is a recombinant antibody developed by antibody phage display technology using a human naive antibody gene library.
Produced without the use of animals.
Purified from HEK 293 cell culture supernatant.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
抗体種類:Mab
宿主:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography from animal component-free supernatant. ≥95% (SDS-PAGE)
Form:Supplied as a liquid in PBS. No preservative added.
Specificity:Recognizes LPS from Legionella pneumophila.
Clone # USB:PHK121-H2 (recomb)
Isotype:IgG1
Calc Applications Abbrev:E WB
Immunogen:Legionella pneumophila (Strain Corby). Selection on whole cell.