HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion.
Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41.
In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding.
In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure.
Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry.
The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
This is a recombinant antibody developed by antibody phage display technology using a human naive antibody gene library.
Produced without the use of animals.
Purified from HEK 293 cell culture supernatant.
Applications:Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
抗体種類：Mab
宿主：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from animal component-free supernatant. ≥95% (SDS-PAGE)
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes gp41 from HIV. Targets the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
