83-5979-25　Cdh2 (Neural Cadherin, N-Cadherin) 50ug　048729

特徴

• The cadherin superfamily of cell adhesion molecules regulate cell-cell interaction and are key factors in developmental morphogenesis and tissue repair.
• Cadherin-2 (N-cadherin; Cdh2), expressed in the vertebrate nervous system, is a type I transmembrane glycoprotein expressed in fusion to an N-terminal propeptide, necessary to avoid intracellular interaction of the molecule.
• Mature Cdh2 consists of five extracellular immunoglobulin (Ig)-like domains (Cdh2-ECDs), a single-pass transmembrane region and an intracellular domain for the interaction with the cell cytoskeleton, mainly mediated by β-catenin binding.
• Throughout the early developmental stages of vertebrates, Cdh2 is expressed almost ubiquitously.
• Later stages its expression seems to be restricted to the central nervous system (CNS) in specific neuronal populations, but it also persists in cardiac muscle.
• In the central and peripheral nervous systems, Cdh2-dependent cell-cell interactions mediate a variety of neurodevelopmental processes including tissue architecture formation, neuronal migration, axon outgrowth, target recognition and synaptogenesis.
• Cdh2 levels are often increased during cancer invasion and metastatic behavior resulting in enhanced cell motility.
• For its role in tumor progression, Cdh2 has been considered a potential biomarker for the prediction of metastases and as an important prognostic factor.
• As a member of the cadherin superfamily of proteins, zebrafish Cdh2 (zCdh2) shares a high degree of amino acid homology with other members of this family.
• Zebrafish (Danio rerio) Cdh2-ECD has an overall amino acid identity of 63% to zebrafish Cadherin-4-ECD, with a peak of 68% in domains 1.
• The Ig-like structure of the 5 extracellular domains and the high sequence similarity to other cadherins requires a careful process of antibody generation against the zCdh2-ECD to avoid unwanted cross-reactivities and a thorough process of binding specificity characterization.
• This is a recombinant antibody developed by antibody phage display technology using a human naive antibody gene library.
• Produced without the use of animals.
• Purified from HEK 293 cell culture supernatant.
• Applications:Suitable for use in Immunohistochemistry, ELISA, Flow Cytometry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.