83-5979-21　Calretinin 100ul　048722

特徴

• Calretinin, as the name suggests, was originally isolated in the retina but was found to be also expressed in mammalian central nerve system, testis, fallopian tube and pancreas.
• It is a cytoplasmic Calcium binding protein which includes typical “EF hand” structures the prototype for which is the protein parvalbumin.
• In the brain calretinin it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types.
• It is particularly concentrated in some cerebellar granular cells and their parallel fibres, but is also found in many GABAergic interneurons in the cortex.
• These GABAergic interneurons in most cases express only one of three related Calcium binding proteins, namely calretinin, calbindin or parvalbumin.
• As a result these important inhibitory interneurons can be identified and classified based on their content of these three proteins.
• Each type of neuron as defined in this fashion has distinct electrophysiological and functional properties.
• Calretinin deficiency in the mossy cells of the mouse dentate gyrus and granule cells results in abnormal excitability in the cerebellar neuronal network and impairment of long-term potentiation and motor coordination, suggesting that calretinin functions as a general Calcium buffer..
• Applications:Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:1000-1:2000Immunofluorescence (IC): 1:1000-1:2000Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.