83-5979-19　Calbindin 50ul　048721

特徴

• Calbindin, also known as calbindin 1 or calbindin-D28k is a member of the large superfamily of cytoplasmic EF hand containing Calcium binding proteins and is expressed in the brain, intestine, kidney and pancreas.
• It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cerebral cortex.
• These GABAergic interneurons in most cases express only one of three Calcium binding proteins, namely calbindin or parvalbumin or calretinin.
• As a result these important and physiologically distinct inhibitory interneurons can be identified and subclassified based on their content of these three proteins.
• This antibody binds calbindin cleanly on western blots and in sections but fails to recognize the related calretinin and parvalbumin proteins.
• It is therefore ideally suited for identifying and subclassifying cortical GABAergic neurons.
• Applications:Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:1000Western Blot: 1:1000-1:5000Immunofluorescence (IC): 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.