83-5979-16　［取扱停止］MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Oncoprotein Mdm2, p53-binding Protein Mdm2) 100ug　048718

特徴

• This gene is a target gene of the transcription factor tumor protein p53.
• The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop.
• Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function.
• This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation.
• This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
• More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
• [provided by RefSeq].
• Applications:Suitable for use in Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml detected MDM2 on 10ug of MCF-7 cell lysate
• Optimal dilutions to be determined by the researcher.
• Positive Control:MCF-7 cell lysate.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.