83-5979-02　Nup98 100ug　048706

特徴

• The human gene NUP98 (Gene ID 4928; Also known as ADAR2, ADIR2, NUP96, NUP196) encodes the nuclear pore complex protein Nup98-Nup96 (UniProt P52948; also known as GLFG-repeat containing nucleoporin, Nup98-Nup96), which is posttranslationally autocleaved to form Nup98 and Nup96.
• Cleavage of the Nup98-96 linkage enhances the interaction between Nup98 and Nup96 and promotes localization of the two proteins to the nuclear pore complex (NPC).
• An alternative splice event generates a mRNA variant (isoform 3; also known as Nup98-specific 1), which expresses only the Nup98 portion of the Nup98-96 mRNA and allows the independent production of Nup98.
• Unlike Nup96, Nup98 is known to exist in both NPC-bound and non-NPC-associated pool, where it may participate in gene transcription regulations.
• Nup98 is reported to interact with histone-modifying enzymes CBP/p300 and histone deacetylases (HDACs) through its glycine-leucine-phenylalanine-glycine (GLFG) repeats.
• NUP98 gene is also involved in chromosomal translocations, resulting in Nup98 translocation fusions identified as potent transcription factors that induce acute myeloid leukemia (AML) with a particularly poor patient prognosis.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml detected Nup98 in 10ug of HeLa nuclear extract
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.