83-5978-90　Uncarboxylated Osteocalcin (Biotin) 100ug　048695

特徴

• Osteocalcin is the most abundant non-collagenous protein found in the bone.
• While fully-carboxylated osteocalcin has a high affinity for the extracellular matrix of the bone; decreases in pH, caused by the process of bone resorption, result in decarboxylation of this protein.
• This generates both partially decarboxylated (undercarboxylated) and fully decarboxylated (uncarboxylated) osteocalcin molecules which are released into the circulation due to decreased affinity for the extracellular matrix.
• Fully carboxylated osteocalcin requires high calcium concentrations for proper protein folding; however, under/uncarboxylated osteocalcin has no calcium requirement to maintain its structure within the blood.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA (detection): 1-4ug/ml, using 048694 as capture antibodyOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.