83-5978-74　beta 2-Microglobulin (B2M) 100ug　048685

特徴

• beta 2-Microglobulin (b2M) is a 12kD secreted polypeptide that serves as the light chain of Class I MHC molecules.
• Possessing an Ig-like domain, b2M noncovalently associates with both 44kD classical (HLA-A, -B, -C) and 40kD non-classical (HLA-E, -F, -G) Class I MHC heavy chains as well as with 43-49kD Class I non-MHC heavy chains (CD1).
• b2M is expressed on nearly all nucleated cells, with neurons being a notable exception.
• Circulating b2M is generated during normal HLA turnover.
• It can also dissociate from the MHC complex and circulate as full length and N-terminal truncated peptides of 93, 91, and 90 amino acids.
• It has been measured in a variety of body fluids, including serum, plasma, saliva, CSF, and urine.
• b2M freely passes through the glomerular membrane, but it is 99% actively reabsorbed and degraded in the proximal tubule cells.
• Circulating b2M levels are elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions with decreased glomerular filtration.
• Human b2M shares 70% and 75% amino acid sequence identity with mouse and rat b2M, respectively.
• Applications:Suitable for use in Flow Cytometry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 2ug/ml under reducing conditionsFlow Cytometry: 0.25ug/10e6 cells in human PBMC lymphocytesOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.