83-5978-72　Mesothelin [KO Validated] (PE) 100Tests　048683

特徴

• Mesothelin is a 40kD glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma.
• Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF).
• Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin.
• Two variant forms exist; variant 1 has an eight amino acid (1kD) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.
• Applications:Suitable for use in Flow Cytometry.
• Other applications have not been tested.
• Recommended Dilutions:Flow Cytometry: 10ul/10e6 cells.
• Validated using HeLa human cervical carcinoma cells.
• KO Validated: Mesothelin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Mesothelin knockout HeLa cell line
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ in the dark.
• DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.