The 90kD heat shock protein (HSP90) is a molecular chaperone that keeps a target protein in a folding-competent state.
Hsp90 is ubiquitously expressed and abundant, and it participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex.
Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.
In its ATP-bound state, Hsp90 interacts with the co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
Hsp90 is highly conserved between species; mammalian proteins demonstrate 60% amino acid identity with the corresponding yeast and 78% identity with the corresponding Drosophila proteins.
Two isoforms of HSP90, alpha and beta, are expressed in the cytosolic compartment.6 The amino acid sequence of human HSP90 alpha is 85% homologous to HSP90 beta, but HSP90 alpha exists predominantly as a homodimer while HSP90 beta exists mainly as a monomer.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: 1:100Western Blot: 1:2500, 1ug/ml was sufficient for detection of HSP90beta in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG (HRP) as the secondary antibody
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes beta isoform of human HSP90 at ~90kD. Species Crossreactivity: chicken, canine, fish, gummy shark (Mustelus antarcticus), hamster, mouse, rabbit, rat, school shark (Galeorhinus galeus), shark and white sucker fish (Catostomus commersonii). Detects HSP90 beta in all reactive species except in chicken, where it detects both alpha and beta isoforms.
Clone # USB：H9010
Isotype：IgG2a
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Ca Ch Hm Hu Mo Rb Rt
Immunogen：Recombinant protein corresponding to human HSP90 beta.
