CD34 is a highly glycosylated monomeric 111-115kD surface protein, which is present on many stem cell populations.
It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible.
CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors.
CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Applications:Suitable for use in Flow Cytometry, Immunocytochemistry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1-2ug/mlImmunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Biotin.
Specificity：Recognizes human CD34. C2386-31D completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Clone # USB：4H11
Isotype：IgG1
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukemia.
