The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell.
The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif.
This motif is composed of four amino acids at the C-terminal end of the protein sequence.
The most common retention sequence is KDEL (lys-asp-glu-leu).
However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6).
There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments.
In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding.
It is also important to note that the KDEL receptor exhibits extensive sequence identity to yeast protein Erd2p, which is a receptor for the yeast ER retention signal .
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000 for colorimetric analysis.
1ug/ml detects KDEL Receptor in 20ug of monkey Vero cell lysate by colorimetric immunoblot assay using an IgG (AP) Pab Gt xMo as the secondary antibody
Immunofluorescence (IC): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
Specificity：Recognizes the bovine KDEL receptor at ~25kD. Species Crossreactivity: human, monkey, mouse, rat, hamster, rabbit, porcine, sheep, canine, chicken, Drosphilia, Xenopus
Clone # USB：9L799 (KR-10)
Isotype：IgG1
Calc Applications Abbrev：FC IC IF IHC IP WB
Calc Crossreactivity：Bo Ca Ch Dr Hm Hu Mk Mo Po Rb Rt Sh Xe
Immunogen：Synthetic peptide corresponding to aa192-212 from the bovine KDEL Receptor and the peptide, conjugated to KLH.
