FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16, and FcγRIV/CD16-2 represent the four known classes of IgG Fc receptors (FcγRs).
FcγRII, FcγRIII, and FcγRIV are low-affinity receptors for monomeric IgG, whereas FcγRI is the only high-affinity FcγR.
FcγRs play important roles in inflammatory cell activation, clearance, presentation of Ag, and maintenance of IgG homeostasis.
In addition to binding immune complex (IC), FcγRs have been shown to bind non-IgG ligands.
For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E.
coli component is reported to negatively regulate the function of macrophage receptor with collagenous structure (MARCO).
FcyRII activation is also reported to transduce a negative regulatory signal against CD38 cross-linking-induced proliferation of resting mature B cells.
Low affinity immunoglobulin gamma Fc region receptor II-a/b/c (IgG Fc receptor II-a/-b/-c, CDw32, Fc-gamma RII-a/-b/-c, Fc-gamma-RIIa/b/c, FcRII-a/-b/-c, CD32) are encoded by the FCGR2A/B/C (CD32, FCG2, IGFR2) genes in human (UniProt P12318, P31994, P31995; Gene ID 2212, 2213, 9103).
The FcγRIIs encoded by the three genes are highly conserved in their extracellular portion, with significant variation in the transmembrane and intracellular regions.
All three types of transcripts alternative splicing to generate additional isoforms.
Applications:Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: detected FcγRIIc immunoreactivity in paraformaldehyde-fixed, Triton X-100-permeabilized human B cell lines carrying the FCGR2C-ORF allele, but not B cell lines with homozygous FCGR2C-STP alleles (Li, X., et al.
(2013).
Sci.
Transl.
Med.
5(216):216ra175)
Immunohistochemistry (Paraffin): 1:50 detected FCGR2A/C in human bone marrow tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human FcγRIIa and FcγRIIc, but not FcγRIIb, by targeting a conserved cytoplasmic domain sequence in human FcγRIIa and FcγRIIc.
Clone # USB：11B6
Isotype：IgG1,k
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Linear peptide corresponding to a cytoplasmic domain sequence identical in human FcγRIIa and FcγRIIc.
